Make sure to sex the pups (you are welcome to post pictures for help if you need it) and separate the males at 3 weeks. You wouldn't want any girls to get pregnant; say the very young male did impregnate any of the girls (sisters or mother), the fact that he and possibly the female(s) are so young and the fact that they are related increase the chances of stillborns/miscarriage and the death of the mother during labor/after giving birth. Make sure you sex them correctly this time.
One is a female and the other is a male, I've already sexed them. I will separate the male once he is 3 weeks old, but how old does he have to be before I can neuter him?
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